Review



amp pnp tocris bioscience  (Tocris)


Bioz Verified Symbol Tocris is a verified supplier
Bioz Manufacturer Symbol Tocris manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Tocris amp pnp tocris bioscience
    Amp Pnp Tocris Bioscience, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amp+pnp/AMP+PNP/pm39818215-841-35-36
    Average 93 stars, based on 6 article reviews
    amp pnp tocris bioscience - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Suspension:

    Article Title: Mitochondrial calcium transport is regulated by P2Y1- and P2Y2-like mitochondrial receptors.
    Article Snippet: Ischemia-reperfusion injury remains a major clinical problem in liver transplantation.. One contributing factor is mitochondrial calcium (mCa2þ) overload, which triggers apoptosis; calcium also regulates mitochondrial respiration and adenosine 50-triphosphate (ATP) production.. Recently, we reported the presence of purinergic P2Y1and P2Y2-like receptor proteins in mitochondrial membranes.

    Transferring:

    Article Title: Regulation of TRPV1 channel activities by intracellular ATP in the absence of capsaicin.
    Article Snippet: Transient receptor potential vanilloid 1 (TRPV1) is a voltage-dependent non-selective cation channel activated by capsaicin, the main pungent ingredient of chili peppers, and noxious heat.. Although TRPV1 channels produce outwardly rectifying currents even in the absence of capsaicin, little is known about the regulation mechanism of the TRPV1 currents.. In the present study, we found that intracellular ATP regulates the basal activities of TRPV1 channels in a concentration-dependent manner.

    Concentration Assay:

    Article Title: Conformational changes in the yeast mitochondrial ABC transporter Atm1 during the transport cycle
    Article Snippet: C-flat 2/2-3C holey carbon-coated copper grids (Science Services ECF-223C-50) were glow discharged in a PELCO easiGlow (Ted Pella Inc.). .. AMP-PNP (TOCRIS #6086) was added 40 min (MSP1D1 data; 3.7 mM final concentration) or 70 min (MSP1E3D1 data; 3.6 mM final concentration) before freezing and incubated on ice. ..

    Incubation:

    Article Title: Conformational changes in the yeast mitochondrial ABC transporter Atm1 during the transport cycle
    Article Snippet: C-flat 2/2-3C holey carbon-coated copper grids (Science Services ECF-223C-50) were glow discharged in a PELCO easiGlow (Ted Pella Inc.). .. AMP-PNP (TOCRIS #6086) was added 40 min (MSP1D1 data; 3.7 mM final concentration) or 70 min (MSP1E3D1 data; 3.6 mM final concentration) before freezing and incubated on ice. ..



    Similar Products

    a620016 amp pnp medchemexpress  (MedChemExpress)
    93
    MedChemExpress a620016 amp pnp medchemexpress
    A620016 Amp Pnp Medchemexpress, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amp+pnp/AMP-PNP/pm40010342-195-57-59
    Average 93 stars, based on 1 article reviews
    a620016 amp pnp medchemexpress - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    8 3h amp pnp  (American Radiolabeled Chemicals Inc)
    86
    American Radiolabeled Chemicals Inc 8 3h amp pnp
    8 3h Amp Pnp, supplied by American Radiolabeled Chemicals Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amp+pnp/3h+8+amp+pnp/pmc12596195-82-3-14
    Average 86 stars, based on 1 article reviews
    8 3h amp pnp - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    nu 406 5 amp pnp jena bioscience  (Jena Bioscience)
    95
    Jena Bioscience nu 406 5 amp pnp jena bioscience
    Nu 406 5 Amp Pnp Jena Bioscience, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amp+pnp/ATP%CE%B3S/pm40961938-286-232-234
    Average 95 stars, based on 1 article reviews
    nu 406 5 amp pnp jena bioscience - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    amp pnp  (Jena Bioscience)
    94
    Jena Bioscience amp pnp
    Amp Pnp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amp+pnp/AppNHp/pmc12339391-577-49-51
    Average 94 stars, based on 1 article reviews
    amp pnp - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    edta treated amp pnp state  (Thermo Fisher)
    99
    Thermo Fisher edta treated amp pnp state
    Edta Treated Amp Pnp State, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amp+pnp/Ethylenediaminetetraacetic+acid/bio_rxiv__2025__07__03__663024-216-12-27
    Average 99 stars, based on 1 article reviews
    edta treated amp pnp state - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    amp pnp  (Merck & Co)
    86
    Merck & Co amp pnp
    Fig. 4. Effect of nucleotide substrates on rHsc70-4 binding to dsRNA and JG-98 treatment on silencing of luciferase. EMSAs testing the binding of rHsc70-4 (2 μM) to dsRNA-Cy3 (dsCat, 0.76 nM) in the presence of (A) ATP-γ-S, (B) <t>AMP-PNP,</t> or (C) ADP. Concentrations of 1, 2.5, 5, and 10 mM of each substrate were used. The electropho- retic shift of dsRNA-Cy3 was assessed by native PAGE. All EMSA experiments were performed at least three times, giving similar results. The first lane in all gels corresponds to dsRNA-Cy3 incubated without rHsc70-4. The last lane in all gels corresponds to dsRNA-Cy3 incubated without rHsc70-4 and with each substrate (10 mM). (D) Effect of pretreatment of S2Xpress cells with a JG-98 inhibitor on silencing of luciferase. Experiments were performed as in Fig. 1C. After transfection with luciferase-expressing plasmids, cells were incubated with JG-98 (0.125, 0.25, 0.5, and 1 μM) or DMSO (1 μM). Then, dsFluc or dsCtl was added to the media. Last, luciferase activity was measured. As positive and negative controls of RNAi silencing, dsFluc and dsCtl were cotransfected, respectively. The histogram shows the mean + SD firefly/Renilla ratio relative to Soak. dsCtl from three independent experiments (n = 9). ANOVA followed by Tukey’s post hoc test was used to detect significant differences between treatments. **P < 0.01; ***P < 0.001; ****P < 0.0001.
    Amp Pnp, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amp+pnp/amp+pnp/pm40378201-431-31-34
    Average 86 stars, based on 1 article reviews
    amp pnp - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    ltag amp pnp mg2  (Thermo Fisher)
    99
    Thermo Fisher ltag amp pnp mg2
    Fig. 4. Effect of nucleotide substrates on rHsc70-4 binding to dsRNA and JG-98 treatment on silencing of luciferase. EMSAs testing the binding of rHsc70-4 (2 μM) to dsRNA-Cy3 (dsCat, 0.76 nM) in the presence of (A) ATP-γ-S, (B) <t>AMP-PNP,</t> or (C) ADP. Concentrations of 1, 2.5, 5, and 10 mM of each substrate were used. The electropho- retic shift of dsRNA-Cy3 was assessed by native PAGE. All EMSA experiments were performed at least three times, giving similar results. The first lane in all gels corresponds to dsRNA-Cy3 incubated without rHsc70-4. The last lane in all gels corresponds to dsRNA-Cy3 incubated without rHsc70-4 and with each substrate (10 mM). (D) Effect of pretreatment of S2Xpress cells with a JG-98 inhibitor on silencing of luciferase. Experiments were performed as in Fig. 1C. After transfection with luciferase-expressing plasmids, cells were incubated with JG-98 (0.125, 0.25, 0.5, and 1 μM) or DMSO (1 μM). Then, dsFluc or dsCtl was added to the media. Last, luciferase activity was measured. As positive and negative controls of RNAi silencing, dsFluc and dsCtl were cotransfected, respectively. The histogram shows the mean + SD firefly/Renilla ratio relative to Soak. dsCtl from three independent experiments (n = 9). ANOVA followed by Tukey’s post hoc test was used to detect significant differences between treatments. **P < 0.01; ***P < 0.001; ****P < 0.0001.
    Ltag Amp Pnp Mg2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amp+pnp/Ampicillin+trihydrate/pm40108462-533-0-15
    Average 99 stars, based on 1 article reviews
    ltag amp pnp mg2 - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    ltag  (Thermo Fisher)
    99
    Thermo Fisher ltag
    Fig. 4. Effect of nucleotide substrates on rHsc70-4 binding to dsRNA and JG-98 treatment on silencing of luciferase. EMSAs testing the binding of rHsc70-4 (2 μM) to dsRNA-Cy3 (dsCat, 0.76 nM) in the presence of (A) ATP-γ-S, (B) <t>AMP-PNP,</t> or (C) ADP. Concentrations of 1, 2.5, 5, and 10 mM of each substrate were used. The electropho- retic shift of dsRNA-Cy3 was assessed by native PAGE. All EMSA experiments were performed at least three times, giving similar results. The first lane in all gels corresponds to dsRNA-Cy3 incubated without rHsc70-4. The last lane in all gels corresponds to dsRNA-Cy3 incubated without rHsc70-4 and with each substrate (10 mM). (D) Effect of pretreatment of S2Xpress cells with a JG-98 inhibitor on silencing of luciferase. Experiments were performed as in Fig. 1C. After transfection with luciferase-expressing plasmids, cells were incubated with JG-98 (0.125, 0.25, 0.5, and 1 μM) or DMSO (1 μM). Then, dsFluc or dsCtl was added to the media. Last, luciferase activity was measured. As positive and negative controls of RNAi silencing, dsFluc and dsCtl were cotransfected, respectively. The histogram shows the mean + SD firefly/Renilla ratio relative to Soak. dsCtl from three independent experiments (n = 9). ANOVA followed by Tukey’s post hoc test was used to detect significant differences between treatments. **P < 0.01; ***P < 0.001; ****P < 0.0001.
    Ltag, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amp+pnp/Ampicillin+trihydrate/pm40108462-533-1-15
    Average 99 stars, based on 1 article reviews
    ltag - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    amp pnp tocris bioscience  (Tocris)
    93
    Tocris amp pnp tocris bioscience
    Fig. 4. Effect of nucleotide substrates on rHsc70-4 binding to dsRNA and JG-98 treatment on silencing of luciferase. EMSAs testing the binding of rHsc70-4 (2 μM) to dsRNA-Cy3 (dsCat, 0.76 nM) in the presence of (A) ATP-γ-S, (B) <t>AMP-PNP,</t> or (C) ADP. Concentrations of 1, 2.5, 5, and 10 mM of each substrate were used. The electropho- retic shift of dsRNA-Cy3 was assessed by native PAGE. All EMSA experiments were performed at least three times, giving similar results. The first lane in all gels corresponds to dsRNA-Cy3 incubated without rHsc70-4. The last lane in all gels corresponds to dsRNA-Cy3 incubated without rHsc70-4 and with each substrate (10 mM). (D) Effect of pretreatment of S2Xpress cells with a JG-98 inhibitor on silencing of luciferase. Experiments were performed as in Fig. 1C. After transfection with luciferase-expressing plasmids, cells were incubated with JG-98 (0.125, 0.25, 0.5, and 1 μM) or DMSO (1 μM). Then, dsFluc or dsCtl was added to the media. Last, luciferase activity was measured. As positive and negative controls of RNAi silencing, dsFluc and dsCtl were cotransfected, respectively. The histogram shows the mean + SD firefly/Renilla ratio relative to Soak. dsCtl from three independent experiments (n = 9). ANOVA followed by Tukey’s post hoc test was used to detect significant differences between treatments. **P < 0.01; ***P < 0.001; ****P < 0.0001.
    Amp Pnp Tocris Bioscience, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amp+pnp/AMP+PNP/pm39818215-841-35-36
    Average 93 stars, based on 1 article reviews
    amp pnp tocris bioscience - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 4. Effect of nucleotide substrates on rHsc70-4 binding to dsRNA and JG-98 treatment on silencing of luciferase. EMSAs testing the binding of rHsc70-4 (2 μM) to dsRNA-Cy3 (dsCat, 0.76 nM) in the presence of (A) ATP-γ-S, (B) AMP-PNP, or (C) ADP. Concentrations of 1, 2.5, 5, and 10 mM of each substrate were used. The electropho- retic shift of dsRNA-Cy3 was assessed by native PAGE. All EMSA experiments were performed at least three times, giving similar results. The first lane in all gels corresponds to dsRNA-Cy3 incubated without rHsc70-4. The last lane in all gels corresponds to dsRNA-Cy3 incubated without rHsc70-4 and with each substrate (10 mM). (D) Effect of pretreatment of S2Xpress cells with a JG-98 inhibitor on silencing of luciferase. Experiments were performed as in Fig. 1C. After transfection with luciferase-expressing plasmids, cells were incubated with JG-98 (0.125, 0.25, 0.5, and 1 μM) or DMSO (1 μM). Then, dsFluc or dsCtl was added to the media. Last, luciferase activity was measured. As positive and negative controls of RNAi silencing, dsFluc and dsCtl were cotransfected, respectively. The histogram shows the mean + SD firefly/Renilla ratio relative to Soak. dsCtl from three independent experiments (n = 9). ANOVA followed by Tukey’s post hoc test was used to detect significant differences between treatments. **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Journal: Science advances

    Article Title: Hsc70-4: An unanticipated mediator of dsRNA internalization in Drosophila .

    doi: 10.1126/sciadv.adv1286

    Figure Lengend Snippet: Fig. 4. Effect of nucleotide substrates on rHsc70-4 binding to dsRNA and JG-98 treatment on silencing of luciferase. EMSAs testing the binding of rHsc70-4 (2 μM) to dsRNA-Cy3 (dsCat, 0.76 nM) in the presence of (A) ATP-γ-S, (B) AMP-PNP, or (C) ADP. Concentrations of 1, 2.5, 5, and 10 mM of each substrate were used. The electropho- retic shift of dsRNA-Cy3 was assessed by native PAGE. All EMSA experiments were performed at least three times, giving similar results. The first lane in all gels corresponds to dsRNA-Cy3 incubated without rHsc70-4. The last lane in all gels corresponds to dsRNA-Cy3 incubated without rHsc70-4 and with each substrate (10 mM). (D) Effect of pretreatment of S2Xpress cells with a JG-98 inhibitor on silencing of luciferase. Experiments were performed as in Fig. 1C. After transfection with luciferase-expressing plasmids, cells were incubated with JG-98 (0.125, 0.25, 0.5, and 1 μM) or DMSO (1 μM). Then, dsFluc or dsCtl was added to the media. Last, luciferase activity was measured. As positive and negative controls of RNAi silencing, dsFluc and dsCtl were cotransfected, respectively. The histogram shows the mean + SD firefly/Renilla ratio relative to Soak. dsCtl from three independent experiments (n = 9). ANOVA followed by Tukey’s post hoc test was used to detect significant differences between treatments. **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Article Snippet: For nucleotide substrate assays, the labeled probe (0.76 nM) was incubated with rHsc70- 4 protein (2 μM) and increasing concentrations of ATP- γ- S (11162306001, Sigma- Aldrich), ADP (A2754, Merck), or AMP- PNP (10102547001, Merck) in PBS.

    Techniques: Binding Assay, Luciferase, Clear Native PAGE, Incubation, Transfection, Expressing, Activity Assay